Collaborative Protein Technology Resource (CPTR)

Is an open core supported by the Office of Science and Technology Resources (OSTR) dedicated to establishing and implementing cutting-edge molecular profiling technologies to facilitate discovery, translational and clinical research.

Project Management
Publications

Technologies

At the Nanoscale Protein Analysis section of CPTR, we offer expertise and provide state-of-the-art technologies to accommodate the needs of CCR/NCI/NIH investigators.

Simple Western

An automated capillary immunoassay system for precise and accurate measurement of proteins and respective post-translational modifications in a format that is applicable to very small sample sizes.

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Luminex xMAP

A multiplex in-solution ELISA analysis system with small sample consumption. The system provides highly quantitative assays for measurement of multiple cytokines, metabolites, and serum/plasma biomarkers in the same sample.

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CODEX (CO-Detection by indEXing)

An immunofluorescence imaging platform for highly multiplexed (30-50 plex) IHC-analysis with single-cell resolution and retention of spatial context. It allows comprehensive in-situ protein profiling with multi-parametric readouts from a single tissue section.

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Digital Spatial Profiling (DSP)

Combines standard immunofluorescence techniques with the nCounter® digital optical barcoding technology to perform highly multiplexed, spatially resolved tissue profiling experiments.

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Single Cell Western

Milo is the world’s first Single Cell Western blotting platform that measures protein expression in ~1000 single cells per run using conventional Western blot antibodies.

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Our Team

Noemi Kedei, MD

Facility Head/Staff Scientist

NIH Building 37, Room 1044

Phone: 240-760-6922

kedein@mail.nih.gov

Xiaoling Luo, MS

Biologist

NIH Building 37, Room 1044

Phone: 240-760-6932

xiaoling.luo@nih.gov

Maria Hernandez, Ph.D

Biologist

NIH Building 37, Room 1044

Phone: 240-760-7774

maria.hernandez2@nih.gov
Address:

37 Convent Drive

Bldg:

37 , Room 1044