Milo: Single Cell Platform
Milo™ is the world’s first Single-Cell Western platform. He measures protein expression in thousands of single cells in a single run so you can profile heterogeneity in your samples. Just load your cell suspension and the scWest chip captures ~1,000 single-cells. Milo then does a fast, 1 minute SDS-PAGE separation on each single-cell lysate on-chip. Then just probe with your favorite conventional Western antibodies to measure ~12 proteins per cell using a variety of multiplexing strategies.
How Can It Help?
Milo unlocks the single-cell proteome and measure more of the proteome than is single-cell technique.
Offers a tool for studying single cell signaling and for dissecting population heterogeneity
Performs Western analysis on 1000-2000 single cells in parallel to quantify expression heterogeneity
Measures proteins that are hard-to-detect by FACS or other single cell analysis technologies, such as isoforms, post-translational modifications, transcription factors, intracellular proteins, etc.
Simple workflow, quantitative data analysis with Scout Software, multiplexing ability depending on the targets (up to 12-15 targets), option to re-probe archived samples months later
Potential Applications
Milo is a powerful tool that can measure the expression of many protiens