CODEX (CO-Detection by indEXing)

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The impact of spatial biology on biomarker research

The spatial architecture of tissue samples can strongly influence disease pathology, progression, and treatment response. Several recent studies have outlined the importance of studying the spatial context of tumor samples to predict treatment response.

Traditional immunohistochemistry (IHC) preserves spatial context but is limited to 2 to 3 biomarkers per sample. Next-generation sequencing (NGS) enables the analysis of multiple biomarkers but the spatial context of the tissue is lost. Multiplex imaging (multiplex IHC) addresses these limitations by enabling the analysis of multiple biomarkers in a tissue section while preserving their spatial context.

The CPTR core implements this high-plex tissue profiling technology to study tumor heterogeneity and microenvironment.

The Technology

The CODEX technology uses antibodies conjugated to a proprietary library of oligonucleotides called Barcodes. This enables customizable panels of up to 40+ CODEX Antibodies to be combined for a single tissue staining reaction.

Advantages of CODEX Technology

Provides full spatial context and is not limited to just regions of interest (ROI)

Provides single-cell resolution down to 600 nm or 250 nm depending on microscope objective used (20X and 40X respectively)

Single-step staining and gentle fluorophore removal preserves the sample for ROI analysis

Simple, benchtop fluidics system that is cost-effective and simple to implement in any research lab